Overview

  • Product name
    Anti-Laminin gamma 1 antibody
    See all Laminin gamma 1 primary antibodies
  • Description
    Rabbit polyclonal to Laminin gamma 1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from the internal region of human Laminin gamma 1.

  • Positive control
    • Extracts from HUVEC cells

Properties

Applications

Our Abpromise guarantee covers the use of ab69632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 178 kDa).
ELISA 1/10000.

Target

  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity
    Found in the basement membranes (major component).
  • Sequence similarities
    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMB2 antibody
    • lamc1 antibody
    • LAMC1_HUMAN antibody
    • Laminin B2 chain antibody
    • Laminin subunit gamma-1 antibody
    • Laminin, gamma 1 (formerly LAMB2) antibody
    • Laminin-1 subunit gamma antibody
    • Laminin-10 subunit gamma antibody
    • Laminin-11 subunit gamma antibody
    • Laminin-2 subunit gamma antibody
    • Laminin-3 subunit gamma antibody
    • Laminin-4 subunit gamma antibody
    • Laminin-6 subunit gamma antibody
    • Laminin-7 subunit gamma antibody
    • Laminin-8 subunit gamma antibody
    • Laminin-9 subunit gamma antibody
    • MGC87297 antibody
    • S-LAM gamma antibody
    • S-laminin subunit gamma antibody
    see all

Images

  • All lanes : Anti-Laminin gamma 1 antibody (ab69632) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 178 kDa
    Observed band size : >170 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Little GH  et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012). WB ; Human . Read more (PubMed: 22187159) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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