Overview

  • Product name
    Anti-Laminin gamma 1 antibody [BC17]
    See all Laminin gamma 1 primary antibodies
  • Description
    Mouse monoclonal [BC17] to Laminin gamma 1
  • Specificity
    The antibody recognizes the gamma 1 chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of clone BC17 has been confirmed by immunoprecipitation and ELISA by using recombinant Laminin gamma 1.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Pepsin-digested laminin from human placenta.

  • General notes
    Balb/c spleen cells used to produce hybridoma.


    The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.
  • Clonality
    Monoclonal
  • Clone number
    BC17
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity
      Found in the basement membranes (major component).
    • Sequence similarities
      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain
      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    References

    This product has been referenced in:
    • Geberhiwot T  et al. Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains. Matrix Biol 19:163-7 (2000). Read more (PubMed: 10842099) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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