Overview

  • Product nameAnti-Laminin gamma 1 antibody [BC17]
    See all Laminin gamma 1 primary antibodies
  • Description
    Mouse monoclonal [BC17] to Laminin gamma 1
  • SpecificityThe antibody recognizes the gamma 1 chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of clone BC17 has been confirmed by immunoprecipitation and ELISA by using recombinant Laminin gamma 1.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Pepsin-digested laminin from human placenta.

  • General notesBalb/c spleen cells used to produce hybridoma.


    The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThe monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.
  • ClonalityMonoclonal
  • Clone numberBC17
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificityFound in the basement membranes (major component).
    • Sequence similaritiesContains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    References for Anti-Laminin gamma 1 antibody [BC17] (ab41823)

    This product has been referenced in:
    • Geberhiwot T  et al. Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains. Matrix Biol 19:163-7 (2000). Read more (PubMed: 10842099) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"