• Product nameAnti-Laminin gamma 1 antibody [SPM277]
    See all Laminin gamma 1 primary antibodies
  • Description
    Rat monoclonal [SPM277] to Laminin gamma 1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Tissue/ cell preparation. (Murine EHS laminin preparation)

  • Positive control
    • LS174T cells. Normal colon or colon carcinoma.



Our Abpromise guarantee covers the use of ab17792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.
IP Use at 2 µg/mg of lysate.
IHC-P 1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.


  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityFound in the basement membranes (major component).
  • Sequence similaritiesContains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMB2 antibody
    • lamc1 antibody
    • LAMC1_HUMAN antibody
    • Laminin B2 chain antibody
    • Laminin subunit gamma-1 antibody
    • Laminin, gamma 1 (formerly LAMB2) antibody
    • Laminin-1 subunit gamma antibody
    • Laminin-10 subunit gamma antibody
    • Laminin-11 subunit gamma antibody
    • Laminin-2 subunit gamma antibody
    • Laminin-3 subunit gamma antibody
    • Laminin-4 subunit gamma antibody
    • Laminin-6 subunit gamma antibody
    • Laminin-7 subunit gamma antibody
    • Laminin-8 subunit gamma antibody
    • Laminin-9 subunit gamma antibody
    • MGC87297 antibody
    • S-LAM gamma antibody
    • S-laminin subunit gamma antibody
    see all

Anti-Laminin gamma 1 antibody [SPM277] images

  • ab17792 at 1/50 staining Laminin gamma 1 from human tonsil by immunohistochemistry
  • ab17792 staining Laminin gamma 1 in dog skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone. Samples were incubated with the primary antibody at a 1/100 dilution for 8 hours at 4°C. An FITC-conjugated goat polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

References for Anti-Laminin gamma 1 antibody [SPM277] (ab17792)

This product has been referenced in:
  • Fournier B  et al. Blockade of the activin receptor IIb activates functional brown adipogenesis and thermogenesis by inducing mitochondrial oxidative metabolism. Mol Cell Biol 32:2871-9 (2012). Read more (PubMed: 22586266) »
  • Steenhard BM  et al. Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. PLoS One 6:e23926 (2011). WB ; Mouse . Read more (PubMed: 21915268) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney)
Permeabilization Yes - 15 min with 0.1% Triton
Specification Kidney
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted May 24 2016

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Acetone
Permeabilization No

Abcam user community

Verified customer

Submitted Oct 19 2010