Overview

  • Product nameAnti-Laminin gamma 1 antibody [SPM277]
    See all Laminin gamma 1 primary antibodies
  • Description
    Rat monoclonal [SPM277] to Laminin gamma 1
  • Tested applicationsICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Tissue/ cell preparation. (Murine EHS laminin preparation)

  • Positive control
    • LS174T cells. Normal colon or colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab17792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.
IP Use at 2 µg/mg of lysate.
IHC-P 1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityFound in the basement membranes (major component).
  • Sequence similaritiesContains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMB2 antibody
    • LAMC1 antibody
    • LAMC1_HUMAN antibody
    • Laminin B2 chain antibody
    • Laminin subunit gamma-1 antibody
    • Laminin, gamma 1 (formerly LAMB2) antibody
    • Laminin-1 subunit gamma antibody
    • Laminin-10 subunit gamma antibody
    • Laminin-11 subunit gamma antibody
    • Laminin-2 subunit gamma antibody
    • Laminin-3 subunit gamma antibody
    • Laminin-4 subunit gamma antibody
    • Laminin-6 subunit gamma antibody
    • Laminin-7 subunit gamma antibody
    • Laminin-8 subunit gamma antibody
    • Laminin-9 subunit gamma antibody
    • MGC87297 antibody
    • S-LAM gamma antibody
    • S-laminin subunit gamma antibody
    see all

Anti-Laminin gamma 1 antibody [SPM277] images

  • ab17792 at 1/50 staining Laminin gamma 1 from human tonsil by immunohistochemistry
  • ab17792 staining Laminin gamma 1 in dog skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone. Samples were incubated with the primary antibody at a 1/100 dilution for 8 hours at 4°C. An FITC-conjugated goat polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

References for Anti-Laminin gamma 1 antibody [SPM277] (ab17792)

This product has been referenced in:
  • Fournier B  et al. Blockade of the activin receptor IIb activates functional brown adipogenesis and thermogenesis by inducing mitochondrial oxidative metabolism. Mol Cell Biol 32:2871-9 (2012). Read more (PubMed: 22586266) »
  • Steenhard BM  et al. Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. PLoS One 6:e23926 (2011). WB ; Mouse . Read more (PubMed: 21915268) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Acetone
Permeabilization No
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Submitted Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"