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Tissue/ cell preparation. (Murine EHS laminin preparation)
Our Abpromise guarantee covers the use of ab17792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.|
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"