Overview

  • Product nameAnti-LAMP1 antibody [1D4B]
    See all LAMP1 primary antibodies
  • Description
    Rat monoclonal [1D4B] to LAMP1
  • SpecificityMurine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa)
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Other, Flow Cyt, IP, IHC-Fr, WB, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Plasma membrane fraction of mouse embryo NIH3T3 cell line.

  • Positive control
    • IHC-P: human lung FFPE tissue sections

Applications

Our Abpromise guarantee covers the use of ab25245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 17215451
Other Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
AP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
Immunomicroscopy Use at an assay dependent concentration.

Target

  • FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similaritiesBelongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody [1D4B] images

  • IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25245, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab25245 staining LAMP1 in Mouse epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with methanol/acetone 1:1 and blocked with 1% milk in PNB for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) in PNB for 16 hours at 4°C. An Alexa Fluor® 568-conjugated Donkey polyclonal to rat IgG, dilution 1/500, was used as secondary antibody. Nuclei were stained blue.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab25245 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25245, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab25245 staining mouse macrophage, hepatoma cell by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 594 conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

References for Anti-LAMP1 antibody [1D4B] (ab25245)

This product has been referenced in:
  • Gaebler A  et al. A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J Lipid Res 57:1934-1947 (2016). Read more (PubMed: 27565170) »
  • Neumann K  et al. Chemokine Transfer by Liver Sinusoidal Endothelial Cells Contributes to the Recruitment of CD4+ T Cells into the Murine Liver. PLoS One 10:e0123867 (2015). ICC/IF ; Mouse . Read more (PubMed: 26052942) »

See all 20 Publications for this product

Product Wall

Thank you for contacting Abcam.

I have heard back from the about whether some of our LAMP1 antibodies recognize an extracellular epitope or not.

Although for ab25630 & ab25245, we have not performed epitope mapping; we have dete...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain (microgliosis neocortex))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization Yes - Tween20
Specification brain (microgliosis neocortex)
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Feb 10 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse macrophage)
Permeabilization No
Specification mouse macrophage
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Mouse Cell (Neuronal progenitor cell line)
Specification Neuronal progenitor cell line
Permeabilization Yes - PBS 1% BSA 0,1% Saponine
Fixative Paraformaldehyde
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Submitted Apr 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Murine macrophage cell line)
Specification Murine macrophage cell line
Fixative Paraformaldehyde
Permeabilization Yes - 10min in PBS + Triton 0.1%
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 15°C
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Submitted Jul 06 2012

Thank you for contacting Abcam regarding ab25245.

I can confirm that according to SwissProt, the predicted molecular weight of LAMP1 is indeed ˜40kDa. However, this protein is subject to glycosylation at numerous site which accounts for the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Epithelial cells)
Specification Epithelial cells
Fixative Methanol/acetone 1:1
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

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Submitted Nov 18 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton in 1%BSA-PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Dr. testtube sibs

Verified customer

Submitted Jan 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (spleen)
Loading amount 1e+006 cells
Specification spleen
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Submitted Jun 24 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Macrophage, hepatoma)
Specification Macrophage, hepatoma
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Tian Xia

Verified customer

Submitted Feb 26 2008

1-10 of 11 Abreviews or Q&A

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