Overview

  • Product nameAnti-LAMP1 antibody
    See all LAMP1 primary antibodies
  • Description
    Rabbit polyclonal to LAMP1
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-KRSHAGYQTI

    , corresponding to amino acids 407-416 of Human LAMP1

  • Positive control
    • HeLa cells. IHC-P: FFPE human lung tissue sections.

Applications

Our Abpromise guarantee covers the use of ab19294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      modifications
      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Anti-LAMP1 antibody images

    • IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • ab19294 has been successfully used in immunofluorescence procedures. ab19294 shows perinuclear staining in HeLa cells (used at 5 µg/ml). The staining is shown in red. Nuclear staining is shown in blue.

    References for Anti-LAMP1 antibody (ab19294)

    This product has been referenced in:
    • Wavre-Shapton ST  et al. Photoreceptor phagosome processing defects and disturbed autophagy in retinal pigment epithelium of Cln3?ex1-6 mice modelling juvenile neuronal ceroid lipofuscinosis (Batten disease). Hum Mol Genet 24:7060-74 (2015). Mouse . Read more (PubMed: 26450516) »
    • Deisl C  et al. Sodium/hydrogen exchanger NHA2 is critical for insulin secretion in ß-cells. Proc Natl Acad Sci U S A 110:10004-9 (2013). Read more (PubMed: 23720317) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your e-mail and your enquiry about our LAMP1 antibodies. Please find below the answers to your questions: Question 1: which polyclonal one works better? Sure you want to say your products all work great, but which one works greater? T...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Monocyte-derived Dendritic Cells)
    Specification Monocyte-derived Dendritic Cells
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.2%
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa epithelial)
    Specification HeLa epithelial
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10%
    Username

    Dr. L Knodler

    Verified customer

    Submitted Mar 22 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"