Overview

  • Product name
  • Description
    Rabbit polyclonal to LAMP1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-KRSHAGYQTI

    , corresponding to amino acids 407-416 of Human LAMP1

  • Positive control
    • HeLa cells. IHC-P: FFPE human lung tissue sections.

Applications

Our Abpromise guarantee covers the use of ab19294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • Sequence similarities
      Belongs to the LAMP family.
    • Post-translational
      modifications
      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • Cellular localization
      Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Images

    • IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • ab19294 has been successfully used in immunofluorescence procedures. ab19294 shows perinuclear staining in HeLa cells (used at 5 µg/ml). The staining is shown in red. Nuclear staining is shown in blue.

    References

    This product has been referenced in:
    • Wavre-Shapton ST  et al. Photoreceptor phagosome processing defects and disturbed autophagy in retinal pigment epithelium of Cln3?ex1-6 mice modelling juvenile neuronal ceroid lipofuscinosis (Batten disease). Hum Mol Genet 24:7060-74 (2015). Mouse . Read more (PubMed: 26450516) »
    • Deisl C  et al. Sodium/hydrogen exchanger NHA2 is critical for insulin secretion in ß-cells. Proc Natl Acad Sci U S A 110:10004-9 (2013). Read more (PubMed: 23720317) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Thank you for your e-mail and your enquiry about our LAMP1 antibodies. Please find below the answers to your questions: Question 1: which polyclonal one works better? Sure you want to say your products all work great, but which one works greater? T...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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