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Anti-LAMP1 antibody (ab24170)

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Overview

Product name

Anti-LAMP1 antibody
See all LAMP1 products (21) ...

Description

Rabbit polyclonal to LAMP1

Tested applications

IP, IHC-P, ICC, ICC/IF, WB, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Zebrafish

Predicted to work with

Cow

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human LAMP1.

(Peptide available as ab25744.)

Positive control

This antibody gave a positive result in the following whole cell lysates: Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) MCF7 (Human breast adenocarcinoma cell line)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170) image (enlarge)

  • Western blot - LAMP1 antibody (ab24170)Western blot - LAMP1 antibody (ab24170) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab24170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

Sequence similarities

Belongs to the LAMP family.

Post-translational
modifications

O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.

Cellular localization

Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Target information above from: UniProt accessionP11279 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD107 antigen like family member A antibody
  • CD107 antigen-like family member A antibody
  • CD107a antibody
  • CD107a antigen antibody
  • LAMP 1 antibody
  • LAMP-1 antibody
  • LAMP1 antibody
  • LAMP1_HUMAN antibody
  • LAMPA antibody
  • LGP120 antibody
  • lgpA antibody
  • Lysosomal membrane glycoprotein 120KD antibody
  • Lysosomal Associated Membrane Protein 1 antibody
  • Lysosome associated membrane glycoprotein 1 antibody
  • Lysosome-associated membrane glycoprotein 1 antibody
  • Lysosome-associated membrane protein 1 antibody
  • OTTHUMP00000040663 antibody
see all

Anti-LAMP1 antibody images:

  Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)

Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)

ICC/IF image of ab24170 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24170, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  Western blot - LAMP1 antibody (ab24170)

Western blot - LAMP1 antibody (ab24170)

All lanes : Anti-LAMP1 antibody (ab24170) at 1 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 120 kDa
Observed band size : 120 kDa
Additional bands at : 23 kDa,35 kDa,45 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170)

ab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information.

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

See Abreview

  Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)

Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)

ab24170 staining LAMP1 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. The cells were PFA fixed, permeabilised in 0.1% Triton X-100 and incubated with the primary antibody at 1µg/ml for 1 hour at 20°C. The secondary antibody used was a donkey anti-rabbit (H+L) IgG conjugated to Alexa Fluor® 555 used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).

This image is courtesy of an anonymous abreview.

See Abreview

  Western blot - LAMP1 antibody (ab24170)

Western blot - LAMP1 antibody (ab24170)

Anti-LAMP1 antibody (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg

Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 120 kDa
Observed band size : 120 kDa


Exposure time : 5 minutes

Blocking Step: 5% Milk for 1 hour at 20°C

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody (ab24170)

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody (ab24170)

ICC/IF image of ab24170 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LAMP1 antibody (ab24170)

This product has been referenced in:

  • Russo Cet al. Small molecule Toll-like receptor 7 agonists localize to the MHC class II loading compartment of human plasmacytoid dendritic cells. Blood 117:5683-91 (2011).Read more (PubMed: 21487111) »
  • Lacruz RSet al. Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling. J Cell Physiol : (2011).Read more (PubMed: 21809343) »

See all 16 publications for this product

Publishing research using ab24170? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"