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Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)
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Western blot - LAMP1 antibody (ab24170)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170)
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Product Name
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LAMP1 antibody
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See all LAMP1 antibodies (19)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to LAMP1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human LAMP1. (Peptide available as ab25744.)
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Reacts with
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Hu, Ms, Rat, Hm
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Predicted to react
(species key)
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Cow, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive result in the following whole cell lysates:
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK293 (Human embryonic kidney cell line)
MCF7 (Human breast adenocarcinoma cell line)
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Cellular localization
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Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
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Research areas
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Cancer >> Cancer Metabolism >> Autophagy
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.6 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.90 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LAMP1 antibody images, references, products related to ab24170 and other tools.
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LAMP1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab24170)
ICC/IF image of ab24170 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24170, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - LAMP1 antibody (ab24170)
All lanes : LAMP1 antibody (ab24170) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 120 kDa
Additional bands at : 23 kDa,35 kDa,45 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP1 antibody (ab24170)
ab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information. This image is courtesy of an Abreview submitted by Mr Carl Hobbs
See Abreview
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References for LAMP1 antibody (ab24170)
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This product has been used in: (two most recent references)
Panyasrivanit M et al. Co-localization of constituents of the dengue virus translation and replication machinery with amphisomes. J Gen Virol 90:448-56 (2009). ICC/IF; Human. PubMed: 19141455
Khakpoor A et al. A role for autophagolysosomes in dengue virus 3 production in HepG2 cells. J Gen Virol 90:1093-103 (2009). PubMed: 19264601
See all 10 publication references for this product.
If you publish research using ab24170 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to LAMP1
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LAMP1 antibody - more information
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