Overview

  • Product name
  • Description
    Rabbit polyclonal to LAMP1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the centre of human LAMP1.

  • Positive control
    • EL4 cell lysate. Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localization
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Images

  • Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
    Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate


    Predicted band size : 45 kDa
  • Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.

  • Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi
  • Immunofluorescence of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.

References

This product has been referenced in:
  • Clement CC  et al. The Dendritic Cell Major Histocompatibility Complex II (MHC II) Peptidome Derives from a Variety of Processing Pathways and Includes Peptides with a Broad Spectrum of HLA-DM Sensitivity. J Biol Chem 291:5576-95 (2016). Read more (PubMed: 26740625) »
  • Abramowski D  et al. Transgenic expression of intraneuronal Aß42 but not Aß40 leads to cellular Aß lesions, degeneration, and functional impairment without typical Alzheimer's disease pathology. J Neurosci 32:1273-83 (2012). ICC/IF ; Mouse . Read more (PubMed: 22279212) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100 in TBS for 10 minutes
Specification
mouse hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Feb 14 2017

The immunogen sequence is unfortunately proprietary information. The laboratory however made an alignement to predict crossreactivity. There is 66.7% homology with bovine [* ** * ** **** ]. The accession number used for the alignment was NP_001068592.1...

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