Anti-LAMP1 antibody (ab62562)
Key features and details
- Rabbit polyclonal to LAMP1
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LAMP1 antibody
See all LAMP1 primary antibodies -
Description
Rabbit polyclonal to LAMP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 15 amino acid peptide from near the centre of human LAMP1.
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Positive control
- ICC: Human colon cells. WB: Mouse EL4 cell lysate. IHC-P: Rat colon tissue and human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
Western blot for mouse only |
Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa). Western blot for mouse only |
Target
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- SwissProt: P14562 Rat
- Unigene: 494419 Human
see all -
Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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Immunocytochemical analysis of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.
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Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
All lanes : EL4 cell lysate
Predicted band size: 45 kDa -
Immunohistochemical analysis of LAMP-1 in rat colon tissue using ab62562 at 20µg/ml.
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Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.
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Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab62562 has been referenced in 20 publications.
- Zhu Y et al. Targeting Aβ and p-Tau Clearance in Methamphetamine-Induced Alzheimer's Disease-Like Pathology: Roles of Syntaxin 17 in Autophagic Degradation in Primary Hippocampal Neurons. Oxid Med Cell Longev 2022:3344569 (2022). PubMed: 35633882
- Hyroššová P et al. Glycosylation defects, offset by PEPCK-M, drive entosis in breast carcinoma cells. Cell Death Dis 13:730 (2022). PubMed: 36002449
- Meecham A et al. Ligand-bound integrin αvβ6 internalisation and trafficking. Front Cell Dev Biol 10:920303 (2022). PubMed: 36092709
- Zhou L et al. Transcription factor EB-mediated autophagy promotes dermal fibroblast differentiation and collagen production by regulating endoplasmic reticulum stress and autophagy-dependent secretion. Int J Mol Med 47:547-560 (2021). PubMed: 33416091
- Hung C et al. SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network. Cell Rep 35:109259 (2021). PubMed: 34133918