Overview

  • Product name
    Anti-LAMP1 antibody (Cy3 ®)
    See all LAMP1 primary antibodies
  • Description
    Rabbit polyclonal to LAMP1 (Cy3 ®)
  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Woodchuck
  • Immunogen

    Synthetic peptide:

    GRKRSHAGYQTI

    conjugated to KLH, corresponding to C terminal amino acids 405-416 of Human LAMP1.

  • Positive control
    • Human Hela cells, rat NRK cells and mouse 3T3 cells.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Molar Ratio (F/P): 3-9.

Properties

Applications

Our Abpromise guarantee covers the use of ab67283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localization
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Images

  • Ab67283 staining LAMP1 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% saponin.  Rabbit Anti-PMP70, Atto 488 Conjugate (peroxisomes stained in green) and 2 μg/mL Rabbit Anti-LAMP1, Cy3 Conjugate (ab67283) (lysosomes stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

  • Ab67283 (2μg) staining LAMP1 in NIH-3T3 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde. 

References

This product has been referenced in:
  • Li D  et al. An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions. J Virol 90:8720-8 (2016). Read more (PubMed: 27440895) »
  • Heinonen MT  et al. Tubulin- and actin-associating GIMAP4 is required for IFN-? secretion during Th cell differentiation. Immunol Cell Biol 93:158-66 (2015). IF . Read more (PubMed: 25287446) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Cultured Retinal Amacrine cells)
Permeabilization
Yes - Saponin
Specification
Cultured Retinal Amacrine cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Dr. Wesley Maddox

Verified customer

Submitted Aug 04 2015



I am happy to inform you that this has now been added to our catalogue: Anti-LAMP1 antibody (ab187185)

Thank you for your message. I apologize for the delay of mine! 1) There are no particular requirements for antibodies to work in flow cytometry, so the mentioned antibodies should give positive results using this technique. It is more important to o...

Read More

Thank you very much for your message. As I myself have been in the very same position, I very well understand that you and your supervisor hesitate to test our different CD107a antibodies. At that time I had the permission by my supervisor to test v...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up