Anti-LAMP1 antibody - Drosophila Lysosome Marker (ab30687)

Overview

  • Product nameAnti-LAMP1 antibody - Drosophila Lysosome Marker
    See all LAMP1 primary antibodies
  • Description
    Rabbit polyclonal to LAMP1 - Drosophila Lysosome Marker
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) LAMP1.

    (Peptide available as ab32341.)

  • Positive control
    • Schneider L2 (Drosophila melanogaster) Whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120-140 kDa).

Detects a band of approximately 120 kDa (predicted molecular weight: 35 kDa for the core protein but an expected size of 120-140 kDa due to glycosylation).

Target

  • FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similaritiesBelongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Alternative names
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody - Drosophila Lysosome Marker images

  • Anti-LAMP1 antibody - Drosophila Lysosome Marker (ab30687) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120-140 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30687 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ab30687 staining LAMP1 in fruit fly (Drosophilia melanogaster) S2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS for 10 minutes and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-LAMP1 antibody - Drosophila Lysosome Marker (ab30687) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 120-140 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-LAMP1 antibody - Drosophila Lysosome Marker (ab30687)

This product has been referenced in:
  • Liu M  et al. The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. Elife 5:N/A (2016). IF ; Drosophila melanogaster . Read more (PubMed: 27536874) »
  • Hicks L  et al. Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology. Biol Open 4:1448-61 (2015). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 26459243) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Fruit fly (Drosophila melanogaster) Cell (S2 Drosophila cells)
Specification S2 Drosophila cells
Permeabilization Yes - 0,25% triton in PBS for 10mins
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Fruit fly (Drosophila melanogaster) Cell (larval fat body)
Specification larval fat body
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 19 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (wing disc)
Specification wing disc
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Po-Lin Chen

Verified customer

Submitted May 10 2013

DISCOUNT CODE: xxxxxx
Expiration date: 22nd January 2013

I am very pleased to hear you would like to accept our offer and test ab30687 in IF. This code will give you: 1 freeprimary antibody or the value of ab30687 off any product in our c...

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The anti-LAMP1 antibody (ab30687) has been tested with Schneider L2 whole cell lysate in Western blotting and found to detect the expected band at ˜130 kDa. We have however as yet...

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Thank you for contacting us. Because we carry over 77,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarant...

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