Overview

  • Product name
    Anti-LAMP1 antibody [LY1C6]
    See all LAMP1 primary antibodies
  • Description
    Mouse monoclonal [LY1C6] to LAMP1
  • Tested applications
    Suitable for: WB, IP, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster
  • Immunogen

    Rat liver lysosomal membrane preparations

  • Positive control
    • Highly purified rat lysosomal membrane preparations.
  • General notes
    For maximum product recovery centrifuge the product vial before removing cap.

Applications

Our Abpromise guarantee covers the use of ab13523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.
IP Use at an assay dependent dilution.
ICC 1/100 - 1/750.
ICC/IF Use at an assay dependent concentration. PubMed: 21536677

Target

  • Function
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localization
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Images

  • Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.

    ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
    WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.
  • ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.

References

This product has been referenced in:
  • Wang B  et al. Essential control of mitochondrial morphology and function by chaperone-mediated autophagy through degradation of PARK7. Autophagy 12:1215-28 (2016). Read more (PubMed: 27171370) »
  • Sun H  et al. Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget 7:76238-76249 (2016). WB ; Rat . Read more (PubMed: 27780926) »

See all 17 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample
Monkey Cell (cos7)
Specification
cos7
Permeabilization
Yes - 2% TritonX100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 13 2014

Thank you for your enquiry and your interest in our products. I believe my colleague has already provided a quote for you. Regarding the immunostaining - ab13523 is an unconjugated primary antibody so you would need a compatible seconda...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (BHK 21)
Specification
BHK 21
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.2%
Username

Dr. Giuseppe Balistreri

Verified customer

Submitted Apr 05 2007

I think your protocol is very good and very similar to the one I have used for ICC, and have been investigating what successful methods were used with ab13523 in ICC. I conclude that I think the problem lies in the fixation step and would like to sugge...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Dog Cell lysate - whole cell (MDCK cell line)
Specification
MDCK cell line
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Mrs. Kun Liu

Verified customer

Submitted Sep 02 2005

Thank you for your enquiry and for providing more information about your protocol. This antibody cross-reacts with rat and hamster LAMP1 but it has not been tested in other species. Unfortunately, we do not have any data indicating its suitability ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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