Overview

  • Product nameAnti-LAMP2 antibody [ABL-93]
    See all LAMP2 primary antibodies
  • Description
    Rat monoclonal [ABL-93] to LAMP2
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse LAMP2.

  • Positive control
    • ICC/IF: RAW246.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IF Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.

Target

  • FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similaritiesBelongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • FormAlternative splicing produces 3 isoforms.
  • Alternative names
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP110 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

Anti-LAMP2 antibody [ABL-93] images

  • All lanes : Anti-LAMP2 antibody [ABL-93] (ab25339) at 1/1000 dilution

    Lane 1 : MEF1 lysate
    Lane 2 : RAW264.7 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase labelled goat anti-rat IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Additional bands at : 120 kDa (possible post-translational modification),90 kDa (possible post-translational modification).

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes.  After transfer, the membrane was blocked for an hour in 2% BSA before being incubated overnight at 4°C with a rat monoclonal [ABL-93] to LAMP2 (ab25339; diluted 1:1000). Antibody binding was detected using peroxidase labelled goat anti-rat IgG (diluted 1:10000) for an hour at room temperature and visualised using ECL development solution (20 minutes exposure).

  • ab25339 staining LAMP2 from mouse liver cells by Immunohistochemistry (Frozen sections). The fresh tissue was cut into small pieces, put into OCT, and frozen in liquid nitrogen. 5µm sections were prepared using a cyrostat. The tissue section was fixed in 5% paraformaldehyde and blocked in 5% serum with 0.05% saponin to increase the permeability. The sample was incubated with ab25339 (1/100 dilution) overnight, and incubated with secondary antibody (Cy2 conjugated donkey anti-rat IgG) for 2 hours. After staining with DAPI (1/1000 dilution) for 2 minutess, the section was mounted.

    See Abreview

  • Overlay histogram showing RAW 264.7 cells stained with ab25339 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25339, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunofluorescence analysis of mouse A20 B cells, staining LAMP2 with ab25339.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 5% FCS in PBS) for 24 hours at 4°C. A FITC-conjugated goat anti-rat polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab25339 stained RAW246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25339, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LAMP2 antibody [ABL-93] (ab25339)

This product has been referenced in:
  • Wu B  et al. Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1. Oncotarget 7:2175-88 (2016). Mouse . Read more (PubMed: 26741505) »
  • Dametto P  et al. Neurodegeneration and unfolded-protein response in mice expressing a membrane-tethered flexible tail of PrP. PLoS One 10:e0117412 (2015). IF ; Mouse . Read more (PubMed: 25658480) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast)
Permeabilization Yes - 0.5% TX-100
Specification Mouse Embryonic Fibroblast
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. Samantha O'Hara

Verified customer

Submitted Dec 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (A20 B cells)
Specification A20 B cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Marcella Flores

Verified customer

Submitted Jan 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (mouse liver cells)
Specification mouse liver cells
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 20 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"