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Purified preparation of mouse liver lysosomal membranes.
Our Abpromise guarantee covers the use of ab13524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. (PMID 2142158).|
|ICC||1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.|
|ICC/IF||1/100. (see Abreview)|
|WB||Use at an assay dependent concentration. See Abreview.|
Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.
ab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview.
Blocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.