Overview

  • Product nameAnti-LAMP2 antibody [GL2A7]
    See all LAMP2 primary antibodies
  • Description
    Rat monoclonal [GL2A7] to LAMP2
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, IP, ICC, ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit
  • Immunogen

    Purified preparation of mouse liver lysosomal membranes.

  • Positive control
    • mouse astrocytes
  • General notesFor maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Properties

Applications

Our Abpromise guarantee covers the use of ab13524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. (PMID 2142158).
ICC 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.
ICC/IF 1/100. (see Abreview)
WB Use at an assay dependent concentration. See Abreview.
IHC-FoFr 1/100. (PubMed:17425404)

Target

  • FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similaritiesBelongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • FormAlternative splicing produces 3 isoforms.
  • Alternative names
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP110 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

Anti-LAMP2 antibody [GL2A7] images

  • Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.

  • ab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg

    Secondary
    Goat anti-rat HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview.

    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

    See Abreview

References for Anti-LAMP2 antibody [GL2A7] (ab13524)

This product has been referenced in:
  • Marcó S  et al. Progressive neurologic and somatic disease in a novel mouse model of human mucopolysaccharidosis type IIIC. Dis Model Mech 9:999-1013 (2016). IHC-P ; Mouse . Read more (PubMed: 27491071) »
  • Kubli DA  et al. PINK1 Is Dispensable for Mitochondrial Recruitment of Parkin and Activation of Mitophagy in Cardiac Myocytes. PLoS One 10:e0130707 (2015). FM ; Mouse . Read more (PubMed: 26110811) »

See all 31 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.1% saponin
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 27 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount 30 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Insu Kwon

Verified customer

Submitted Dec 10 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Aplysia Tissue sections (ganglia)
Specification ganglia
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

Submitted Dec 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Uterus)
Permeabilization No
Specification Uterus
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 4 µg
Gel Running Conditions Reduced Denaturing (4-12)
Sample Mouse Tissue lysate - whole (uterus)
Specification uterus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 24 2013

Thank you for your enquiry.

I have obtained some more details regarding these antibodies which I hope will be helpful:

ab13524 Anti-LAMP2 antibody [GL2A7] The immunogen used to generate this antibody was purified mouse liver lysos...

Read More

Thank you for your patience while we looked into this further. I'll be helping you until Caitlin comes back in on Monday.

The supplying laboratory doesn't test this antibody for WB, only in IP. In IP the bands are usually around 100-120kDa. T...

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Thank you for your enquiry.

There are are few types of Saponin (Gypsophila saponins, soyasaponin I, or saponins extracted from lucerne (Medicago sativa) plant tops) available but the idea here is to use it as a gentle detergent, from a pract...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2012

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"