• Product name
    Anti-LAMP2 antibody [GL2A7]
    See all LAMP2 primary antibodies
  • Description
    Rat monoclonal [GL2A7] to LAMP2
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Fr, IP, ICC, ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit
  • Immunogen

    Purified preparation of mouse liver lysosomal membranes.

  • Positive control
    • mouse astrocytes
  • General notes
    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.



Our Abpromise guarantee covers the use of ab13524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. (PMID 2142158).
ICC 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.
ICC/IF 1/100. (see Abreview)
WB Use at an assay dependent concentration. See Abreview.
IHC-FoFr 1/100. (PubMed:17425404)


  • Function
    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificity
    Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in disease
    Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localization
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Form
    Alternative splicing produces 3 isoforms.
  • Alternative names
    • CD107 antigen like family member B antibody
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP 96 antibody
    • LGP110 antibody
    • LGP96 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane glycoprotein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all


  • Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.

  • ab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg

    Goat anti-rat HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)

    Exposure time: 3 minutes

    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

    See Abreview


This product has been referenced in:

See all 54 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

I have obtained some more details regarding these antibodies which I hope will be helpful:

ab13524 Anti-LAMP2 antibody [GL2A7] The immunogen used to generate this antibody was purified mouse liver lysos...

Read More

Thank you for your patience while we looked into this further. I'll be helping you until Caitlin comes back in on Monday.

The supplying laboratory doesn't test this antibody for WB, only in IP. In IP the bands are usually around 100-120kDa. T...

Read More

Thank you for your enquiry.

There are are few types of Saponin (Gypsophila saponins, soyasaponin I, or saponins extracted from lucerne (Medicago sativa) plant tops) available but the idea here is to use it as a gentle detergent, from a pract...

Read More

Thank you for your enquiry. As far as we know, the epitope of ab13524 is not determinded yet. I hope this information helps you, and please do not hesitate to contact us if you have further questions.

I'm sorry to hear you are having a problem with ab13524 and ab18180. I think the problem you are experiencing with the LAMP2 antibody may be due to the fixative, the antibody works only with methanol fixed cells or PLP (paraformaldehyde-lysine-peri...

Read More

Thank you for your reply. I am very sorry to hear that even the replacement vial failed to work. Looking at our order record in the computer system, I can see that we have sold a lot of vials of this particular product and our customer feedback ind...

Read More

Thank you for your recent phone call regarding the location of the epitopes recognized by ab2733, ab2900 and ab13524. As discussed on the phone ab2733 is known to bind the extracellular domain of the Mannose 6 Phospate Receptor. For ab2900 the immu...

Read More

Thank you for your enquiry and for providing more information about your protocol. I am very sorry to hear that you are having problem with this antibody. As the datasheet indicates, this antibody has only been tested for ICC but not for IHC. I...

Read More


Sign up