Overview

  • Product name
    Anti-LAMP3 antibody
    See all LAMP3 primary antibodies
  • Description
    Rabbit polyclonal to LAMP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44, 64 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
  • Tissue specificity
    Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
  • Sequence similarities
    Belongs to the LAMP family.
  • Cellular localization
    Lysosome membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD208 antibody
      • CD208 antigen antibody
      • DC LAMP antibody
      • DC lysosome associated membrane glycoprotein antibody
      • DC-lysosome-associated membrane glycoprotein antibody
      • DCLAMP antibody
      • LAMP antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • Lamp3 antibody
      • LAMP3_HUMAN antibody
      • Lysosomal associated membrane protein 3 antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome-associated membrane glycoprotein 3 antibody
      • Protein TSC403 antibody
      • TSC403 antibody
      see all

    Images

    • Western blot - LAMP3 antibody (ab83659)
      Western blot - LAMP3 antibody (ab83659)
      Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44,64 kDa (why is the actual band size different from the predicted?)



      Gel concentration 12%

    References

    ab83659 has not yet been referenced specifically in any publications.

    Publishing research using ab83659? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Good afternoon, I bought an antibody from abcam, Anti-LAMP3 antibody (ab83659) I did a western blot using SH-SY5Y whole cell lysate, and I got two bands, one between 37 and 50 kDa and other between 50 and 75 kDa. I see...

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    Thank you for contacting us. LAMP3 is glycosylated and the higher MW band is likely a glycosylated form of the protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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