Overview

  • Product name
    Anti-LAP2 alpha antibody [3A3]
    See all LAP2 alpha primary antibodies
  • Description
    Mouse monoclonal [3A3] to LAP2 alpha
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human LAP2 alpha.

    Read Abcam's proprietary immunogen policy

    . Please note that the peptide contains a Cys at position 690 instead of Arg. However, the 3A3 antibody recognizes wt LAP2 alpha and mutant LAP2 alpha equally well.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates. IF/ICC: HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab66588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).

Target

  • Relevance
    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Isoforms beta/gamma antibody
      • Lamina associated polypeptide 2 antibody
      • LAP2 antibody
      • Thymopoietin antibody
      • Thymopoietin isoform alpha antibody
      • TMPO antibody
      • TP alpha antibody
      see all

    Images

    • Western blot - Anti-LAP2 alpha antibody [3A3] (ab66588)
      Western blot - Anti-LAP2 alpha antibody [3A3] (ab66588)
      All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 75 kDa
      Observed band size: 77 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody [3A3] (ab66588)
      Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody [3A3] (ab66588)
      ICC/IF image of ab66588 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

    References

    This product has been referenced in:
    • Qi R  et al. The lamin-A/C-LAP2a-BAF1 protein complex regulates mitotic spindle assembly and positioning. J Cell Sci 128:2830-41 (2015). Read more (PubMed: 26092935) »

    See 1 Publication for this product

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