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Overview

  • Product name
    Anti-LAP2 alpha antibody [3A3]
    See all LAP2 alpha primary antibodies
  • Description
    Mouse monoclonal [3A3] to LAP2 alpha
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human LAP2 alpha.

    Read Abcam's proprietary immunogen policy

    . Please note that the peptide contains a Cys at position 690 instead of Arg. However, the 3A3 antibody recognizes wt LAP2 alpha and mutant LAP2 alpha equally well.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates. IF/ICC: HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab66588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).

Target

  • Relevance
    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Isoforms beta/gamma antibody
      • Lamina associated polypeptide 2 antibody
      • LAP2 antibody
      • Thymopoietin antibody
      • Thymopoietin isoform alpha antibody
      • TMPO antibody
      • TP alpha antibody
      see all

    Anti-LAP2 alpha antibody [3A3] images

    • Western blot - LAP2 alpha antibody [3A3] (ab66588)
      Western blot - LAP2 alpha antibody [3A3] (ab66588)
      All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 75 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    • Immunocytochemistry/ Immunofluorescence - LAP2 alpha antibody [3A3] (ab66588)
      Immunocytochemistry/ Immunofluorescence - LAP2 alpha antibody [3A3] (ab66588)
      ICC/IF image of ab66588 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

    References for Anti-LAP2 alpha antibody [3A3] (ab66588)

    ab66588 has not yet been referenced specifically in any publications.

    Publishing research using ab66588? Please let us know so that we can cite the reference in this datasheet.

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