Overview

  • Product nameAnti-LAP2 alpha antibody [3A3]
    See all LAP2 alpha primary antibodies
  • Description
    Mouse monoclonal [3A3] to LAP2 alpha
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human LAP2 alpha.

    . Please note that the peptide contains a Cys at position 690 instead of Arg. However, the 3A3 antibody recognizes wt LAP2 alpha and mutant LAP2 alpha equally well.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates. IF/ICC: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab66588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).

Target

  • RelevanceLamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Isoforms beta/gamma antibody
    • Lamina associated polypeptide 2 antibody
    • LAP2 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • TMPO antibody
    • TP alpha antibody
    see all

Anti-LAP2 alpha antibody [3A3] images

  • All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 75 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66588 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References for Anti-LAP2 alpha antibody [3A3] (ab66588)

ab66588 has not yet been referenced specifically in any publications.

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