Anti-LAP2 alpha antibody (ab5162)
Key features and details
- Rabbit polyclonal to LAP2 alpha
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LAP2 alpha antibody
See all LAP2 alpha primary antibodies -
Description
Rabbit polyclonal to LAP2 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human LAP2 alpha aa 188-693.
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General notes
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eu karyotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/2500. Detects a band of approximately 83 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/2500. Detects a band of approximately 83 kDa. |
Target
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Relevance
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Entrez Gene: 25359 Rat
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: Q61033 Mouse
- SwissProt: Q62733 Rat
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Alternative names
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
Images
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ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.
A = ab5162, 1/250
B = DNA
C = A and B merged
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ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL). ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab5162 has been referenced in 23 publications.
- Harhouri K et al. MG132 Induces Progerin Clearance and Improves Disease Phenotypes in HGPS-like Patients' Cells. Cells 11:N/A (2022). PubMed: 35203262
- Maurizi E et al. Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity. Int J Mol Sci 23:N/A (2022). PubMed: 35628669
- Ding B et al. Disease Modeling with Human Neurons Reveals LMNB1 Dysregulation Underlying DYT1 Dystonia. J Neurosci 41:2024-2038 (2021). PubMed: 33468570
- Zhu X et al. Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation. Br J Pharmacol 178:3428-3447 (2021). PubMed: 33864382
- Vaz M & Fahrenkrog B The Retinoblastoma Tumor Suppressor Is Required for the NUP98-HOXA9-Induced Aberrant Nuclear Envelope Phenotype. Cells 10:N/A (2021). PubMed: 34831074