Overview

  • Product nameAnti-LAP2 antibody [RL29]
    See all LAP2 primary antibodies
  • Description
    Armenian Hamster monoclonal [RL29] to LAP2
  • SpecificityDetects Lamin Associated Polypeptide (LAP) 2 from various rat tissues but does not cross-react with LAP 1A, 1B & 1C. This antibody recognizes a single 53 kDa protein representing LAP 2 in rat liver nuclear envelope fractions.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) corresponding to Rat LAP2. This is a Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

Properties

Applications

Our Abpromise guarantee covers the use of ab2738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • RelevanceLamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Lamina associated polypeptide 2 antibody
    • LAP 2 antibody
    • LEM domain containing 4 antibody
    • LEMD4 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • Thymopoietin related peptide isoform alpha antibody
    • Thymopoietin related peptide isoforms beta/gamma antibody
    • Thymopoietin, isoforms beta/gamma antibody
    • TMPO antibody
    • TP alpha antibody
    • TP antibody
    • TP beta/gamma antibody
    • TPRP isoform alpha antibody
    • TPRP isoforms beta/gamma antibody
    see all

References for Anti-LAP2 antibody [RL29] (ab2738)

This product has been referenced in:
  • Li Z  et al. Werner complex deficiency in cells disrupts the Nuclear Pore Complex and the distribution of lamin B1. Biochim Biophys Acta 1833:3338-45 (2013). Read more (PubMed: 24050918) »
  • Foisner R & Gerace L Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73:1267-79 (1993). Read more (PubMed: 8324822) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. This product was produced from a hybridoma derived from spleen cells of an Armenian hamster immunized with pore comlex-lamina fraction isolated from rat liver. Therefore, for the secondary antibody, an Armenian IgG would...

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We have not performed studies to determine which isoform of LAP2 our antibody binds. Therefore I am unable to give specific information on this.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"