Overview

  • Product name
  • Description
    Rabbit polyclonal to LARG
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1350 and 1400 of human LARG (NP_056128.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 173 kDa (predicted molecular weight: 173 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
  • Tissue specificity
    Ubiquitously expressed. Isoform 2 is found in jejunum and testis.
  • Involvement in disease
    A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 PH domain.
    Contains 1 RGSL (RGS-like) domain.
  • Cellular localization
    Cytoplasm. Membrane. Translocated to the membrane upon stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGC_HUMAN antibody
    • ARHGEF12 antibody
    • KIAA0382 antibody
    • leukemia-associated rho guanine nucleotide exchange factor antibody
    • Leukemia-associated RhoGEF antibody
    • PRO2792 antibody
    • Rho guanine exchange factor (GEF) 12 antibody
    • Rho guanine nucleotide exchange factor (GEF) 12 antibody
    • Rho guanine nucleotide exchange factor 12 antibody
    • Similar to Mouse Lsc oncogene antibody
    see all

Images

  • All lanes : Anti-LARG antibody (ab86095) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 173 kDa
    Observed band size : 173 kDa


    Exposure time : 3 minutes
  • Detection of LARG by Western Blot of Immunprecipitate.
    ab86095 at 1µg/ml staining LARG in HeLa whole cell lysate immunoprecipitated using ab86095 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Osborne LD  et al. TGF-ß regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion. Mol Biol Cell 25:3528-40 (2014). WB . Read more (PubMed: 25143398) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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