The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 173 kDa (predicted molecular weight: 173 kDa).
Use at 2-5 µg/mg of lysate.
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Ubiquitously expressed. Isoform 2 is found in jejunum and testis.
Involvement in disease
A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1.
All lanes : Anti-LARG antibody (ab86095) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 173 kDa Observed band size: 173 kDa
Exposure time: 3 minutes
Immunoprecipitation - LARG antibody (ab86095)
Detection of LARG by Western Blot of Immunprecipitate.
ab86095 at 1µg/ml staining LARG in HeLa whole cell lysate immunoprecipitated using ab86095 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.