Overview

  • Product nameAnti-LAS1L antibody
    See all LAS1L primary antibodies
  • Description
    Rabbit polyclonal to LAS1L
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse
  • Immunogen

    A region within synthetic peptide: PASSSFGSEA KAQQQEEQGS VNDVKEEEKE EKEVLPDQVE EEEENDDQEE, corresponding to C terminal amino acids 541-590 of Human LAS1L

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab49279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 83 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionInvolved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
  • Sequence similaritiesBelongs to the LAS1 family.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ475B7.2 antibody
    • FLJ12525 antibody
    • FLJ30133 antibody
    • FLJ45274 antibody
    • LAS1 like (S. cerevisiae) antibody
    • Las1 like antibody
    • LAS1L antibody
    • LAS1L_HUMAN antibody
    • Protein LAS1 homolog antibody
    • Ribosomal biogenesis protein LAS1L antibody
    • RP3 475B7.2 antibody
    see all

Anti-LAS1L antibody images

  • Anti-LAS1L antibody (ab49279) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit Ig at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 83 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-LAS1L antibody (ab49279)

ab49279 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (LUNG CANCER)
Loading amount 200000 cells
Specification LUNG CANCER
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jun 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"