Anti-LAS1L antibody - C-terminal (ab190390)

Overview

  • Product name
    Anti-LAS1L antibody - C-terminal
    See all LAS1L primary antibodies
  • Description
    Rabbit polyclonal to LAS1L - C-terminal
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human LAS1L aa 684-734 (C terminal). The exact sequence is proprietary. NP_112483.1.
    Sequence:

    RLEPSTC KTDTLGLSCG VGSGNCSNSS SSNFEGLLWS QGQLHGLKTG LQLF


    Database link: Q9Y4W2

  • Positive control
    • HeLa, 293T, Jurkat, TCMK1, and NIH 3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab190390 was affinity purified using an epitope specific to LAS1L immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 83 kDa.

Target

  • Function
    Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
  • Sequence similarities
    Belongs to the LAS1 family.
  • Cellular localization
    Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ475B7.2 antibody
    • FLJ12525 antibody
    • FLJ30133 antibody
    • FLJ45274 antibody
    • LAS1 like (S. cerevisiae) antibody
    • Las1 like antibody
    • LAS1L antibody
    • LAS1L_HUMAN antibody
    • Protein LAS1 homolog antibody
    • Ribosomal biogenesis protein LAS1L antibody
    • RP3 475B7.2 antibody
    see all

Images

  • All lanes : Anti-LAS1L antibody - C-terminal (ab190390) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 83 kDa


    Exposure time : 30 seconds
  • Detection of LAS1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab190390 at 6 µg/reaction for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 3o seconds.

References

ab190390 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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