Overview

  • Product name
  • Description
    Rabbit polyclonal to LASP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human LASP1 (NP_006139.1).

  • Positive control
    • Whole cell lysate from HeLa, 293T and Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117806 was affinity purified using an epitope specific to LASP1 immobilized on a solid support .
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
    Contains 2 nebulin repeats.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • LASP 1 antibody
    • LASP-1 antibody
    • LASP1 antibody
    • LASP1_HUMAN antibody
    • LIM and SH3 domain protein 1 antibody
    • LIM and SH3 protein 1 antibody
    • Metastatic lymph node gene 50 protein antibody
    • MLN 50 antibody
    • MLN50 antibody
    • OTTHUMP00000164238 antibody
    • OTTHUMP00000164239 antibody
    see all

Images

  • All lanes : Anti-LASP1 antibody (ab117806) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 30 kDa


    Exposure time : 30 seconds
  • ab117806 at 1 µg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab117806 at 6 µg/mg lysate.
    Lane 2; IP using an antibody to a downstream epitope.
    Lane 3; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.

References

This product has been referenced in:
  • Hu JJ  et al. Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells. Proteome Sci 12:13 (2014). WB ; Dog . Read more (PubMed: 24580839) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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