Overview

  • Product name
  • Description
    Goat polyclonal to LASP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig
  • Immunogen

    Synthetic peptide:

    NPNCARCGKIVYP

    , corresponding to N terminal amino acids 2-14 of Human LASP1.

  • Positive control
    • WB: Mouse brain extracts.
  • General notes
    Principal Names: LASP1 (LIM and SH3 protein 1), MLN50. GenBank Accession Number: NP006139 LocusLink ID: 3927 Please note that one of our customers has had problems getting this antibody to detect endogenous Lasp-1 in 2 different human cell lines. Furthermore, a LASP-1/GFP fusion protein overexpressed in a cell line could not be detected with this antibody and yet was detected with our Rabbit Polyclonal to GFP (ab290). For further information please see the enquiries section of the online datasheet. We are currently conducting more QC tests on this product and are interested to hear from other researchers using it. 24 March, 2004.


    This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human LASP1 peptide (ab22798).

Target

Images



  • Predicted band size : 30 kDa


    LASP1 staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

References

This product has been referenced in:
  • Li K  et al. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. J Proteome Res 4:725-33 (2005). Read more (PubMed: 15952719) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (IMR-90 cells)
Loading amount
30 µg
Specification
IMR-90 cells
Gel Running Conditions
Non-reduced Denaturing
Username

Abcam user community

Verified customer

Submitted Apr 22 2009

Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been...

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Thank you for your enquiry. I apologize for the confusion, the antibody is antigen affinity purified: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. If you have any ...

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I have asked our accounts department to refund you for the cost of this antibody. (Order reference 35289). Thank you for the extra information you have provided about the nature of your fusion constructs. We will follow your advice and have this a...

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Your feedback places doubt on the sensitivity/specificity of our antibody and we take it very seriously. As we discussed over the telephone, I will gladly refund you for the cost of this antibody as it clearly has not worked in your research. I'm afrai...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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