Overview

  • Product name
  • Description
    Rabbit polyclonal to LASS3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide derived from a region between amino acids 54-103 of human LASS3.

  • Positive control
    • Foetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-LASS3 antibody (ab28637) at 0.5 µg/ml + Foetal liver lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 46 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 kDa,45 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Kralova J  et al. Glycol porphyrin derivatives and temoporfin elicit resistance to photodynamic therapy by different mechanisms. Sci Rep 7:44497 (2017). Read more (PubMed: 28295025) »
  • Lucki NC  et al. Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression. Mol Endocrinol 26:228-43 (2012). Read more (PubMed: 22261821) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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