Overview

  • Product nameAnti-LAT antibody
    See all LAT primary antibodies
  • Description
    Rabbit polyclonal to LAT
  • SpecificitySpecific for both forms of human LAT (36 and 38kDa).
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 31-233 of Human LAT.

  • Positive control
    • Jurkat lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: 4 µg immunoprecipitates LAT from 500 µg Jurkat RIPA lysate.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa or 38 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
    • Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
    • Post-translational
      modifications
      Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
    • Cellular localizationCell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 36 kDa phospho tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adaptor protein antibody
      • LAT 1 antibody
      • lat antibody
      • LAT_HUMAN antibody
      • LAT1 antibody
      • Linker for activation of T cells antibody
      • Linker for activation of T cells family member 1 antibody
      • linker for activation of T cells, transmembrane adaptor antibody
      • Linker for activation of T-cells family member 1 antibody
      • p36 38 antibody
      • p36-38 antibody
      • pp36 antibody
      see all

    References for Anti-LAT antibody (ab14518)

    ab14518 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"