Overview

  • Product name
  • Description
    Rabbit polyclonal to LAT
  • Specificity
    Binds to a 36-40 Kd protein in normal mouse lung and heart tissue. A larger size band of approx. 40-45 Kd is seen in human tumour cell lines.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    CHRLPGSYDSTSSDSLYPRGI

    , corresponding to amino acids 29-48 of Human LAT.

  • Positive control
    • Human K562 and Jurkat cell lines. Mouse lung and heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC 1/300.

Target

  • Function
    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • Tissue specificity
    Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • Post-translational
    modifications
    Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • Cellular localization
    Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Images

  • Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).

References

This product has been referenced in:
  • Kota DJ  et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes 62:2048-58 (2013). WB . Read more (PubMed: 23349496) »
  • Zikherman J  et al. Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival. Proc Natl Acad Sci U S A 109:E3-12 (2012). WB ; Mouse . Read more (PubMed: 22135465) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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