Overview

  • Product nameAnti-LAT antibody
    See all LAT primary antibodies
  • Description
    Rabbit polyclonal to LAT
  • Specificityab59229 detects endogenous levels of total LAT protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 191 (R-E-YP-V-N).

  • Positive control
    • NIH 3T3 cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • Post-translational
    modifications
    Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • Cellular localizationCell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Anti-LAT antibody images

  • All lanes : Anti-LAT antibody (ab59229) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extract
    Lane 2 : NIH 3T3 cell extract with immunising peptide


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-LAT antibody (ab59229)

ab59229 has not yet been referenced specifically in any publications.

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