Overview

  • Product nameAnti-LAT antibody [Y109]
    See all LAT primary antibodies
  • Description
    Rabbit monoclonal [Y109] to LAT
  • SpecificityThis antibody is specific for LAT. It detects the long and short forms of LAT.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding the GRB2 binding motif 2 of Human LAT.

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human lymph node.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/60.
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
    • Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
    • Post-translational
      modifications
      Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
    • Cellular localizationCell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 36 kDa phospho tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adaptor protein antibody
      • LAT 1 antibody
      • lat antibody
      • LAT_HUMAN antibody
      • LAT1 antibody
      • Linker for activation of T cells antibody
      • Linker for activation of T cells family member 1 antibody
      • linker for activation of T cells, transmembrane adaptor antibody
      • Linker for activation of T-cells family member 1 antibody
      • p36 38 antibody
      • p36-38 antibody
      • pp36 antibody
      see all

    Anti-LAT antibody [Y109] images

    • Anti-LAT antibody [Y109] (ab32070) at 1/500 dilution + Jurkat cell lysate.

      Predicted band size : 28 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • Ab32070, at a dilution of 1/100, staining LAT in paraffin embedded human lymph node tissue by Immunohistochemistry.

    References for Anti-LAT antibody [Y109] (ab32070)

    ab32070 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"