SpecificityDetects endogenous levels of LAT only when phosphorylated at tyrosine 191.
This antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561).
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use at an assay dependent concentration.
FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Post-translational modificationsPhosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
Cellular localizationCell membrane. Present in lipid rafts.
Linker for activation of T cells family member 1 antibody
linker for activation of T cells, transmembrane adaptor antibody
Linker for activation of T-cells family member 1 antibody
p36 38 antibody
Anti-LAT (phospho Y191) antibody images
Immunohistochemistry (Paraffin-embedded sections) - LAT (phospho Y191) antibody (ab59197)
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59197 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.
Western blot - LAT (phospho Y191) antibody (ab59197)
All lanes : Anti-LAT (phospho Y191) antibody (ab59197) at 1/500 dilution
Lane 1 : NIH3T3 cells treated with PMA Lane 2 : NIH3T3 cells treated with PMA with phosphopeptide
Predicted band size : 28 kDa Observed band size : 28 kDa
References for Anti-LAT (phospho Y191) antibody (ab59197)
This product has been referenced in:
Crites TJ et al. TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction. J Immunol193:56-67 (2014).
Read more (PubMed: 24860189) »
Kota DJ et al. TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity. Diabetes62:2048-58 (2013).
Read more (PubMed: 23349496) »