Overview

  • Product nameAnti-LAT (phospho Y220) antibody [E225]
    See all LAT primary antibodies
  • Description
    Rabbit monoclonal [E225] to LAT (phospho Y220)
  • SpecificityThis antibody detects LAT phosphorylated on Tyrosine 191 in isoform 2 (SwissProt: O43561-2), and Tyrosine 220 in isoform 1 (SwissProt: O43561).
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human LAT.

  • Positive control
    • Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-LAT (phospho Y191) antibody (HRP) [E225] (ab199511)

    Anti-LAT (phospho Y191) antibody (Biotin) [E225] (ab199755)

Properties

Applications

Our Abpromise guarantee covers the use of ab32434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 36,38 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50.
ICC 1/50.
IP 1/10.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionRequired for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
    • Tissue specificityExpressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
    • Post-translational
      modifications
      Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
    • Cellular localizationCell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 36 kDa phospho tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adaptor protein antibody
      • LAT 1 antibody
      • lat antibody
      • LAT_HUMAN antibody
      • LAT1 antibody
      • Linker for activation of T cells antibody
      • Linker for activation of T cells family member 1 antibody
      • linker for activation of T cells, transmembrane adaptor antibody
      • Linker for activation of T-cells family member 1 antibody
      • p36 38 antibody
      • p36-38 antibody
      • pp36 antibody
      see all

    Anti-LAT (phospho Y220) antibody [E225] images

    • All lanes : Anti-LAT (phospho Y220) antibody [E225] (ab32434) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with anti-CD3


      Predicted band size : 28 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
      Anti-CD3 was used to induce phosphorylation of LAT at Tyr191.
    • ab32434, at a 1/50 dilution, staining human tonsils by immunohistochemistry, Paraffin embedded tissue.

    References for Anti-LAT (phospho Y220) antibody [E225] (ab32434)

    This product has been referenced in:
    • Wang D  et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat Immunol 13:560-8 (2012). Read more (PubMed: 22561606) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"