Anti-LAT2 antibody [8B12] (ab123896)

Overview

  • Product nameAnti-LAT2 antibody [8B12]
    See all LAT2 primary antibodies
  • Description
    Mouse monoclonal [8B12] to LAT2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human LAT2 protein expressed in 293T cells.

  • Positive control
    • HEK293T cells transfected with the pCMV6-ENTRY LAT2; Human kidney carcinoma tissue.
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab123896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 58 kDa.
IHC-P 1/50.

Target

  • FunctionSodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
  • Tissue specificityStrongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.
  • Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localizationCytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLAT2 antibody
    • integral membrane protein E16H antibody
    • L type amino acid transporter 2 antibody
    • L-type amino acid transporter 2 antibody
    • Large neutral amino acids transporter small subunit 2 antibody
    • LAT2_HUMAN antibody
    • LPI PC1 antibody
    • SLC7A8 antibody
    • solute carrier family 7 (amino acid transporter light chain, L system), member 8 antibody
    • solute carrier family 7 (amino acid transporter, L type), member 8 antibody
    • solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 antibody
    • Solute carrier family 7 member 8 antibody
    see all

Anti-LAT2 antibody [8B12] images

  • All lanes : Anti-LAT2 antibody [8B12] (ab123896) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY LAT2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 58 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204737)
  • ab123896, at 1/50, staining LAT2 in paraffin-embedded Human kidney carcinoma tissue by immunohistochemistry.

References for Anti-LAT2 antibody [8B12] (ab123896)

ab123896 has not yet been referenced specifically in any publications.

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