Anti-Late Inflammatory Macrophages antibody [25 F 9] (FITC) (ab15689)

Overview

  • Product name
    Anti-Late Inflammatory Macrophages antibody [25 F 9] (FITC)
  • Description
    Mouse monoclonal [25 F 9] to Late Inflammatory Macrophages (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig, Rhesus monkey
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • Positive control
    • Tested with subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells.
  • General notes


    Monoclonal antibody 25F9 is associated with fully differentiated tissue macrophages both in normal and diseased tissues, independently of the presence or absence of inflammation. The antibody is also suitable for staining macrophages from bronchial lavage fluids and similar techniques. It is very useful for macrophage phenotyping, particularly for the classification of late inflammatory stages. It is used in tissue sections and in smears, for the characterization of tumorous tissues and the monitoring of macrophage cell cultures.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Monoclonal antibody 25F9 is associated with fully differentiated tissue macrophages both in normal and diseased tissues, independently of the presence or absence of inflammation. The antibody is also suitable for staining macrophages from bronchial lavage fluids and similar techniques. It is very useful for macrophage phenotyping, particularly for the classification of late inflammatory stages. It is used in tissue sections and in smears, for the characterization of tumorous tissues and the monitoring of macrophage cell cultures.
  • Clonality
    Monoclonal
  • Clone number
    25 F 9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

Target

  • Cellular localization
    .
  • Alternative names
    • Late stage inflammatory macrophages antibody

Images

  • ab15689 at 1/150 staining sections of monkey spleen by IHC-P

    The tissue section was incubated with ab15689 for 1 hr at 23°C followed by a secondary incubation with an HRP-conjugated Goat anti-Mouse antibody.  

    The blocking agent was 5% serum, incubated for 20 min at 23°C.

    See Abreview

References

ab15689 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Nov 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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