• Product nameAnti-LATS1 antibody
    See all LATS1 primary antibodies
  • Description
    Rabbit polyclonal to LATS1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human LATS1.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: A549; HCT116; U20S; HeLa.



Our Abpromise guarantee covers the use of ab105106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).


  • FunctionNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • Tissue specificityExpressed in all adult tissues examined except for lung and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational
    Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Anti-LATS1 antibody images

  • All lanes : Anti-LATS1 antibody (ab105106) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 127 kDa
    Observed band size : 127 kDa
    Additional bands at : 24 kDa,50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-LATS1 antibody (ab105106)

ab105106 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Nov 15 2011