Overview

  • Product nameAnti-LATS1 +LATS2 antibody
    See all LATS1 +LATS2 primary antibodies
  • Description
    Rabbit polyclonal to LATS1 +LATS2
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to Human LATS1 +LATS2 (N terminal).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 120 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
  • Cellular localizationCytoplasm; cytoskeleton; centrosome.
  • Database links
  • Alternative names
    • 4932411G09Rik antibody
    • AV277261 antibody
    • AW208599 antibody
    • AW228608 antibody
    • FLJ13161 antibody
    • KPM antibody
    • Large tumor supressor, homolog 1 antibody
    • LATS, large tumor suppressor, homolog 1 (Drosophila) antibody
    • LATS, large tumor suppressor, homolog 2 (Drosophila) antibody
    • RGD1564085 antibody
    • Serine/threonine protein kinase LATS1 antibody
    • Serine/threonine protein kinase LATS2 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Anti-LATS1 +LATS2 antibody images

  • All lanes : Anti-LATS1 +LATS2 antibody (ab70565) at 0.4 µg/ml

    Lane 1 : HeLa cell whole lysate at 50 µg
    Lane 2 : HeLa cell whole lysate at 15 µg
    Lane 3 : HeLa cell whole lysate at 5 µg


    Predicted band size : 120 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of human LATS2 by Immunoprecipitation in HeLa whole cell lysate (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70565 at 3 µg/mg of lysate.
    Subsequent blotting of immunoprecipitate for the detection of LATS2 was performed using 1µg/ml of ab70565. Detection was by chemiluminescence.
  • ab70565 (2µg/ml) staining LATS2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in pneumocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-LATS1 +LATS2 antibody (ab70565)

This product has been referenced in:

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 20 µg
Specification HCT116 cells
Treatment Transfected with empty vector (pCDNA3) or a vector expressing Lats2
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Efrat Shema

Verified customer

Submitted Aug 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 25 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"