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Recombinant full length Human LAYN
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Target information above from: UniProt accession
The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
has not yet been referenced specifically in any publications.
Publishing research using ab89790? Please let us know so that we can cite the reference in this datasheet.
4 Abreviews or Q&A
No, I was using human Jurkat T cell samples and human HT29 colon carcinoma samples, not mouse samples. My only concern was whether the antibody is labeled wrong, and if it is rabbit rather than mouse.
Asked on Mar 16 2012
Thank you for clarifying that information. As requested I have sent you ab88766, a Mouse polyclonal to LAYN as a free of charge replacement for ab89790. Your new order/reference number is ******. Please let me know if there is anyth...
Abcam Scientific Support
Answered on Mar 16 2012
So I repeated this experiment with new samples and a new dilution of the antibody (1:250). Again, there is absolutely no signal whatsoever. I suspect either the antibody is degraded or not actually mouse. Either way, I would like a replacement. The ori...
Thank you for your reply. I would just like to clarify the samples that you were using. Were you using mouse and human cells, or just mouse cells? I ask as we have only tested this antibody on human sample, so we do not know if this antibody ...
Hi, Well, I'm trying the blot again, but I don't have much hope for it. To answer your questions I do the following: The lysates were from the human cell lines Jurkat and AN3CA, loading 20 ug of lysate per lane. <...
Asked on Mar 13 2012
Thank you for your reply and for providing some protocol information. I understand that your may publish many papers using western blotting as a technique and therefore you are very proficient, but we do need to have some information about ho...
Answered on Mar 13 2012
I ordered this antibody last week and have had some issues with it. First off, are you sure it is mouse? I see almost no bands whatsoever with the antibody, even at a 1:250 dilution and using super ECL. If it isn't mouse, that would expl...
Asked on Mar 12 2012
Thank you for contacting Abcam. I can confirm that ab89790 is a mouse monoclonal antibody against LAYNand under our Abpromise we guarantee it to work on human tissue in western blot and if I cannot help resolve the problems you are having, th...
Answered on Mar 12 2012
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"