Overview

  • Product name
  • Description
    Rabbit polyclonal to LBH
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PDYLRSAKMT EVMMNTQPME EIGLSPRKDG LSYQIFPDPS DFDRCCKLKD RLPSIVVEPT EGEVESGELR WPPEEFLVQE DEQDNCEETA KE, corresponding to amino acids 10-101 of Human LBH (UniProt ID: Q53QV2).

  • Positive control
    • IHC-P: Human hippocampus tissue ICC/IF: Human cell line A 431

Properties

Applications

Our Abpromise guarantee covers the use of ab122223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function
    Transcriptional activator which may act in mitogen-activated protein kinase signaling pathway.
  • Tissue specificity
    Highly expressed in heart, and expressed at low levels in placenta, lung, skeletal muscle, kidney and liver.
  • Sequence similarities
    Belongs to the LBH family.
  • Developmental stage
    Expressed in heart of 25 and 17 weeks embryos.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLBH antibody
    • lbh antibody
    • LBH_HUMAN antibody
    • Limb bud and heart development antibody
    • Limb bud and heart development homolog (mouse) antibody
    • Protein LBH antibody
    see all

Images

  • ab122223, at a 1/400 dilution, staining LBH in paraffin embedded Human hippocampus tissue by Immunohistochemistry.
  • ab122223, at 4 µg/ml, staining LBH in Human cell line A-431 by Immunofluorescence. Antibody staining is shown in green.

References

ab122223 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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