The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
FunctionTranscriptional activator which may act in mitogen-activated protein kinase signaling pathway.
Tissue specificityHighly expressed in heart, and expressed at low levels in placenta, lung, skeletal muscle, kidney and liver.
Sequence similaritiesBelongs to the LBH family.
Developmental stageExpressed in heart of 25 and 17 weeks embryos.