Anti-LC3A/B antibody (ab62721)
Key features and details
- Rabbit polyclonal to LC3A/B
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LC3A/B antibody -
Description
Rabbit polyclonal to LC3A/B -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
Sequence:ERYKGEKQLPVLDK
Database link: Q9H492 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB | (3) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 81631 Human
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 67443 Mouse
- Entrez Gene: 362245 Rat
- Entrez Gene: 64862 Rat
- Omim: 601242 Human
- Omim: 609604 Human
see all -
Alternative names
- ATG8F antibody
- Autophagy related protein LC3 A antibody
- Autophagy related protein LC3 B antibody
see all
Images
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Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
Lanes 2-3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml
Lanes 1-2 : Rat whole brain extract
Lane 3 : Rat whole brain extract with immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Predicted band size: 16 - 18 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab62721 has been referenced in 43 publications.
- Huang L et al. Berberine Attenuates IL-1β-Induced Damage of Nucleus Pulposus Cells via Activating the AMPK/mTOR/Ulk1 Pathway. Biomed Res Int 2022:6133629 (2022). PubMed: 35915801
- Ge J et al. TP53-induced glycolysis and apoptosis regulator (TIGAR) ameliorates lysosomal damage in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-mediated mouse model of Parkinson's disease. Toxicol Lett 339:60-69 (2021). PubMed: 33359019
- Shao Z et al. RNA-binding protein HuR suppresses senescence through Atg7 mediated autophagy activation in diabetic intervertebral disc degeneration. Cell Prolif 54:e12975 (2021). PubMed: 33372336
- Mao J et al. ASPP2 expression predicts the prognosis of patients with hepatocellular carcinoma after transcatheter arterial chemoembolization. Exp Ther Med 21:397 (2021). PubMed: 33680119
- Liu G et al. Ginsenoside Rg2 protects cardiomyocytes against trastuzumab-induced toxicity by inducing autophagy. Exp Ther Med 21:473 (2021). PubMed: 33767768