Overview

  • Product name
  • Description
    Rabbit polyclonal to LC3A/B
  • Specificity
    Recognizes human and rat LC3A/B-I and LC3A/B-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively). Recognizes mouse LC3A/B-I (~18 kDa). The detection of mouse LC3A/B-II may require specific cell treatment and/or long exposure.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
    Sequence:

    ERYKGEKQLPVLDK


    Database link: Q9H492

  • Positive control
    • Whole brain. U87 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
IP Use at an assay dependent dilution.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related protein LC3 B antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 B antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3 antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/1B light chain 3 B antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • MAP1LC3A antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 alpha antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule associated proteins 1A/1B light chain 3A antibody
    • Microtubule associated proteins 1A/1B light chain 3B antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
    Lanes 2 - 3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml

    Lane 1 : Rat whole brain extract
    Lane 2 : Rat whole brain extract
    Lane 3 : Rat whole brain extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate
    Developed using the ECL technique

    Predicted band size : 16 - 18 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Wang J  et al. Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy. Aging Dis 8:71-84 (2017). WB . Read more (PubMed: 28203482) »
  • Wang J  et al. Cerebral ischemic post-conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model. Mol Med Rep 14:4162-4172 (2016). WB ; Rat . Read more (PubMed: 27666823) »

See all 20 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
35 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 19 2017

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (COV434 cell line)
Specification
COV434 cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (Muscle)
Specification
Muscle
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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