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Product name Anti-LC3A/B antibody See all LC3A/B primary antibodies
Rabbit polyclonal to LC3A/B
Specificity Recognizes human and rat LC3A/B-I and LC3A/B-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively). Recognizes mouse LC3A/B-I (~18 kDa). The detection of mouse LC3A/B-II may require specific cell treatment and/or long exposure.
Tested applications Suitable for:
WB, IP more details
Mouse, Rat, Human
Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
Database link: Q9H492
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
Use at an assay dependent dilution.
Information by UniProt
Autophagy related protein LC3 A antibody
Autophagy related protein LC3 B antibody
Anti-LC3A/B antibody images
Western blot - Anti-LC3A/B antibody (ab62721)
Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml Lanes 2 - 3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml Lane 1 : Rat whole brain extract Lane 2 : Rat whole brain extract Lane 3 : Rat whole brain extract with immunizing peptide Secondary Goat Anti-Rabbit IgG, Peroxidase conjugate Developed using the ECL technique Predicted band size : 16 - 18 kDa Observed band size : 18 kDa ( why is the actual band size different from the predicted?)
References for Anti-LC3A/B antibody (ab62721)
This product has been referenced in:
et al. Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy. Cardiovasc Ther 34:352-9 (2016).
Read more (PubMed: 27333569) »
et al. Cerebral ischemic post-conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model. Mol Med Rep 14:4162-4172 (2016).
Read more (PubMed: 27666823) »
See all 9 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"