Overview

  • Product nameAnti-LC3A/B antibody
    See all LC3A/B primary antibodies
  • Description
    Rabbit polyclonal to LC3A/B
  • SpecificityRecognizes human and rat LC3A/B-I and LC3A/B-II by immunoblotting and immunoprecipitation (~18 kDa and ~16 kDa, respectively). Recognizes mouse LC3A/B-I (~18 kDa). The detection of mouse LC3A/B-II may require specific cell treatment and/or long exposure.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3A/B aa 36-49 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Also SwissProt ID: Q9GZQ8
    Sequence:

    ERYKGEKQLPVLDK


    Database link: Q9H492

  • Positive control
    • Whole brain. U87 cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab62721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 - 18 kDa).
IP Use at an assay dependent dilution.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related protein LC3 B antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 B antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3 antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/1B light chain 3 B antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • MAP1LC3A antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 alpha antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule associated proteins 1A/1B light chain 3A antibody
    • Microtubule associated proteins 1A/1B light chain 3B antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Anti-LC3A/B antibody images

  • Lane 1 : Anti-LC3A/B antibody (ab62721) at 0.5 µg/ml
    Lanes 2 - 3 : Anti-LC3A/B antibody (ab62721) at 1 µg/ml

    Lane 1 : Rat whole brain extract
    Lane 2 : Rat whole brain extract
    Lane 3 : Rat whole brain extract with immunizing peptide

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate
    Developed using the ECL technique

    Predicted band size : 16 - 18 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References for Anti-LC3A/B antibody (ab62721)

This product has been referenced in:
  • Gao H  et al. Rho-Kinase inhibitor fasudil suppresses high glucose-induced H9c2 cell apoptosis through activation of autophagy. Cardiovasc Ther 34:352-9 (2016). Read more (PubMed: 27333569) »
  • Marino FE  et al. Activin-ßC modulates cachexia by repressing the ubiquitin-proteasome and autophagic degradation pathways. J Cachexia Sarcopenia Muscle 6:365-80 (2015). WB ; Mouse . Read more (PubMed: 26673867) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (COV434 cell line)
Specification COV434 cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Verified customer

Submitted Feb 13 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (Muscle)
Specification Muscle
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"