Western blot abreview for Anti-LC3B antibody

Excellent
Abreviews
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Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Treatment
50µg/kg AEE thrice weekly for 1 week by gavage, RAD 2,5mg/kg daily for 1 week by gavage
Gel Running Conditions
Non-reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk

Secondary antibody

Dilution
1/5000

Detection

Detection method
chemiluminescent
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
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Submitted May 09 2008

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