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Synthetic peptide corresponding to Human LC3B (N terminal). A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).
Database link: Q9GZQ8
Our Abpromise guarantee covers the use of ab48394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration. 20 ug per 500 ug of protein.|
|IHC-P||1/200 - 1/400.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
Detects bands of approximately 17 kDa (LC3-II) and 19 kDa (LC3-I) (predicted molecular weight: 15 kDa).
|IHC-Fr||Use at an assay dependent concentration.|
ab48394 staining LC3B in a Rat hepatocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% Donkey serum in 0.1% PBST for 60 minutes at 21°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA) for 3 hours at 22°C. An Alexa Fluor® 394-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/200.
Immunohistochemistry analysis of (formalin-fixed,paraffin-embedded sections) analysis of human brain,cerebral cortex,neurons with cell processes LC3B with ab48394
Immunocytochemistry/Immunofluorescence analysis of HeLa cells with Dylight 488 (green).Nuclei and alpha -tubulin were counterstained with DAPI (blue) and Dylight 550 (red).