Overview

  • Product nameAnti-LC3B antibody
    See all LC3B primary antibodies
  • Description
    Rabbit polyclonal to LC3B
  • Specificityab51520 is specific for the B isoform of LC3.
  • Tested applicationsIHC - Wholemount, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide made to the N terminal region of human LC3, isoform B protein.

  • Positive control
    • WB: Treated U87-MG lysates. ICC/IF: Treated U87-MG cells. human malignant brain tumors, breast cancer, leukemia samples and murine cells
  • General notes


    ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab51520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration.
IHC-Fr 1/2000.
WB 1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
ICC/IF 1/2000.

Target

  • FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificityMost abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similaritiesBelongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy related protein LC3 B antibody
    • Autophagy related ubiquitin like modifier LC3 B antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1B light chain 3 B antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • MAP1LC3B antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule associated proteins 1A/1B light chain 3B antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Anti-LC3B antibody images

  • All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution

    Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
    Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
    Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
    Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM


    Predicted band size : 15 kDa
  • Ab51520 at 1/2000 dilution staining treated U373-MG cells;
    1) Control
    2) C2-ceramide.
    The nuclei were stained with DAPI.
  • Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
    1) Control
    2) Temozolomide
  • Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
    1) Control
    2) Temozolomide
  • Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
    1) normal brain
    2) gliobastoma multiform tissue

References for Anti-LC3B antibody (ab51520)

This product has been referenced in:
  • Llewellyn KJ  et al. Lipid-enriched diet rescues lethality and slows down progression in a murine model of VCP-associated disease. Hum Mol Genet 23:1333-44 (2014). ICC/IF ; Mouse . Read more (PubMed: 24158850) »
  • Mangieri LR  et al. ATP6V0C knockdown in neuroblastoma cells alters autophagy-lysosome pathway function and metabolism of proteins that accumulate in neurodegenerative disease. PLoS One 9:e93257 (2014). WB . Read more (PubMed: 24695574) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Mouse Cell (RAW cell)
Specification RAW cell
Permeabilization Yes - 3% BSA in PBS with 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

The antibody's immunogen is over 95% identical to the homologous sequence in zebrafish. Since this is a polyclonal antibody, and is assumed to recognize multiple epitopes, it is likely that it recognizes epitopes that are 100% identical in the immunoge...

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Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE gel)
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Treatment HeLa cells without or with 50 uM chloroquine for 18 hrs
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (uterus, placenta)
Loading amount 50 µg
Specification uterus, placenta
Treatment LPS
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Dr. Varkha Agrawal

Verified customer

Submitted May 14 2013

Application IHC - Wholemount
Sample Zebrafish Embryo (Muscle)
Specification Muscle
Username

Abcam user community

Verified customer

Submitted May 02 2013

Thank you for contacting us.

We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. <...

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Thank you for your reply.

I will be pleased to provide an free of charge vial of from a different lot.

Regrettably, this antibody is not in stock at the moment. Another batch is in production and is expected to be available by the...

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Thank you for contacting us.

I am sorry to hear of the issues you have been experiencing. Usually high background can be adjusted by changing your blocking solution. If you have used milk, try BSA or if BSA try milk. However I would recomm...

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Thank you for your call today and for letting us know about the trouble with ab51520.

As we discussed, I'm sending a free of charge replacement vial on the order ***, which should arrive promptly.

Please keep me updated about how th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"