Overview

  • Product name
  • Description
    Rabbit polyclonal to LC3B
  • Specificity
    ab51520 is specific for the B isoform of LC3.
  • Tested applications
    Suitable for: IHC - Wholemount, Flow Cyt, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
    Database link: Q9GZQ8

  • Positive control
    • WB: Treated U87-MG lysates. ICC/IF: Treated U87-MG cells. human malignant brain tumors, breast cancer, leukemia samples and murine cells
  • General notes

    ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.

    The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab51520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt 1/200.
IHC-Fr 1/2000.
WB 1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
ICC/IF 1/2000.

Target

  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity
    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution

    Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
    Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
    Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
    Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM


    Predicted band size : 15 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

  • Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
    1) Control
    2) Temozolomide
  • Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.

  • Ab51520 at 1/2000 dilution staining treated U373-MG cells;
    1) Control
    2) C2-ceramide.
    The nuclei were stained with DAPI.
  • Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
    1) Control
    2) Temozolomide
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

  • Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
    1) normal brain
    2) gliobastoma multiform tissue
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).

References

This product has been referenced in:
  • Wang X  et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15:2097-2105 (2017). Read more (PubMed: 28259912) »
  • Cho JH  et al. Downregulation of SIRT1 signaling underlies hepatic autophagy impairment in glycogen storage disease type Ia. PLoS Genet 13:e1006819 (2017). WB . Read more (PubMed: 28558013) »

See all 54 Publications for this product

Customer reviews and Q&As

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Regrettably, this antibody is not in stock at the moment. Another batch is in production and is expected to be available by the...

Read More
Application
Western blot
Sample
Monkey Tissue lysate - whole (Cardiac)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cardic stem celll)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardic stem celll
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2016

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 03 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 06 2015

Application
Flow Cytometry
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - Triton X-100, 0.01%
Gating Strategy
LC3B-AlexaFluor488 positive
Specification
Cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: 0.25% for 30 sec
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample
Human Cultured Cells (HEK293)
Specification
HEK293
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (RAW cell)
Specification
RAW cell
Permeabilization
Yes - 3% BSA in PBS with 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

1-10 of 23 Abreviews or Q&A

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