Overview

  • Product nameAnti-LC3B antibody (Biotin)
    See all LC3B primary antibodies
  • Description
    Rabbit polyclonal to LC3B (Biotin)
  • ConjugationBiotin
  • SpecificityLC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Zebrafish, Syrian Hamster, Bacteria
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to an N-terminal portion of human LC3 B protein (between residues 1-100).
    Database link: Q9GZQ8

  • Positive control
    • Brain, cerebral cortex. Mouse ES cell lysates. Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies.

Applications

Our Abpromise guarantee covers the use of ab81785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 15 kDa.
IP Use at 40 µg/mg of lysate.
IHC-P 1/200 - 1/400.

Target

  • FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificityMost abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similaritiesBelongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Anti-LC3B antibody (Biotin) images

  • All lanes : Anti-LC3B antibody (Biotin) (ab81785) at 2 µg/ml

    Lane 1 : Atg5+/+ mouse ES cell lysate
    Lane 2 : Atg5-/- mouse ES cell lysate


    Predicted band size : 15 kDa
    Observed band size : 17,19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,40 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    In Western blot, bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I.

  • ab81785, at 1/200 dilution, staining LC3A/B in brain, cerebral cortex, neurons with cell processes by Immunohistochemistry using paraffin-embedded tissue.
  • ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-LC3B antibody (Biotin) (ab81785)

This product has been referenced in:
  • Luo CL  et al. Autophagy is involved in traumatic brain injury-induced cell death and contributes to functional outcome deficits in mice. Neuroscience 184:54-63 (2011). WB, IHC-Fr ; Mouse . Read more (PubMed: 21463664) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step protein block as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Aug 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"