Overview

  • Product name
    Anti-LC3B antibody - N-terminal
    See all LC3B primary antibodies
  • Description
    Rabbit polyclonal to LC3B - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human LC3B aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MPSEKTFKQR RTFEQRVEDV RLIREQHPTK


    Database link: Q9GZQ8

  • Positive control
    • WB: Extracts from untreated or rapamycin-treated cultures of wild-type (LC3B+/+) or homozygous cerebellar cells, 293 cell line lysates transiently transfected with LC3B, Rat brain lysate.
      IF: LSK cells, Rat brain.
      IHC: Human testis tissue and Wild-type (LC3B+/+) or homozygous Mice brain sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab168831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 15 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity
    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8F antibody
    • Autophagy-related protein LC3 B antibody
    • Autophagy-related ubiquitin-like modifier LC3 B antibody
    • LC3B antibody
    • LC3II antibody
    • MAP1 light chain 3 like protein 2 antibody
    • MAP1 light chain 3-like protein 2 antibody
    • MAP1A/1BLC3 antibody
    • MAP1A/MAP1B LC3 B antibody
    • MAP1A/MAP1B light chain 3 B antibody
    • MAP1ALC3 antibody
    • MAP1LC3B a antibody
    • Map1lc3b antibody
    • Microtubule associated protein 1 light chain 3 beta antibody
    • Microtubule-associated protein 1 light chain 3 beta antibody
    • Microtubule-associated proteins 1A/1B light chain 3B antibody
    • MLP3B_HUMAN antibody
    see all

Images

  • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling LC3B with ab168831 at 1/50.
  • Immunofluorescence analysis of FACS sorted LSK cells (Lineage-Sca-1+c-kit+, enriched for hematopoietic stem cells) labeling LC3B with ab168831 at 1/50. An AlexaFlour 488 conjugated anti-rabbit antibody at 1/200 was used as the secondary.
  • Immunofluorescence analysis of Rat brain before (left) and after (right) treatment with rapamycin (100 nm for 4 hr) labeling LC3B with ab168831 at 1/50.
  • All lanes : Anti-LC3B antibody - N-terminal (ab168831) at 1/100 dilution

    Lane 1 : Membrane fraction of Rat brain lysate
    Lane 2 : Soluble fraction of Rat brain lysate

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 15 kDa



    Both non-lipidated (arrow, I) and lipidated LC3B (arrow, II) were detected in membrane fraction (P) but pro-LC3B and non-lipidated LC3B were detected in soluble fraction (S).
  • All lanes : Anti-LC3B antibody - N-terminal (ab168831) at 1/60 dilution

    Lane 1 : 293 cell line lysates transiently transfected with LC3B
    Lane 2 : Non-transfected 293 cell line

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 15 kDa

  • All lanes : Anti-LC3B antibody - N-terminal (ab168831) at 1/100 dilution

    Lane 1 : Untreated LC3B wild-type (CbCln3+/+) cerebellar cells extracts
    Lane 2 : Untreated LC3B homozygous(CbCln3Äex7/8/Äex7/8) cerebellar cells extracts
    Lane 3 : Rapamycin-treated (250 nM, 4 h) LC3B wild-type (CbCln3+/+) cerebellar cells extracts
    Lane 4 : Rapamycin-treated (250 nM, 4 h) LC3B homozygous(CbCln3Äex7/8/Äex7/8) cerebellar cells extracts

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 15 kDa

  • Immunohistochemical analysis of Wild-type (Cln3+/+) or homozygous Cln3Äex7/8 (Cln3Äex7/8/Äex7/8) paraffin-embedded murine brain sections labeling LC3B using ab168831 at a 1/50 dilution.
    KEY: CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer.

References

This product has been referenced in:
  • Cabang AB  et al. Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer. Oncotarget 8:104347-104358 (2017). Read more (PubMed: 29262645) »
  • Lim SW  et al. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Sci Rep 6:29921 (2016). Rat . Read more (PubMed: 27436514) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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