Overview

  • Product nameAnti-LCAT antibody
    See all LCAT primary antibodies
  • Description
    Rabbit polyclonal to LCAT
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 292-321 of Human LCAT

  • Positive control
    • K562 cell line lysates Brain tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab103489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCentral enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
  • Tissue specificityExpressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. In liver, expressed in HEPG2 hepatocytes.
  • Involvement in diseaseDefects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) [MIM:245900]; also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure.
    Defects in LCAT are a cause of fish-eye disease (FED) [MIM:136120]; also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye').
  • Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
  • Post-translational
    modifications
    O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures.
  • Cellular localizationSecreted. Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid.
  • Information by UniProt
  • Database links
  • Alternative names
    • LCAT antibody
    • LCAT_HUMAN antibody
    • Lecithin cholesterol acyltransferase antibody
    • Lecithin-cholesterol acyltransferase antibody
    • Phosphatidylcholine sterol acyltransferase antibody
    • Phosphatidylcholine-sterol acyltransferase antibody
    • Phospholipid cholesterol acyltransferase antibody
    • Phospholipid-cholesterol acyltransferase antibody
    see all

Anti-LCAT antibody images

  • Anti-LCAT antibody (ab103489) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 50 kDa
  • IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab103489 used at 1:50 dilution

References for Anti-LCAT antibody (ab103489)

ab103489 has not yet been referenced specifically in any publications.

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