Overview

  • Product nameAnti-Lck antibody
    See all Lck primary antibodies
  • Description
    Rabbit polyclonal to Lck
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to the C-terminus of human Lck.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab18896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 58 kDa.Can be blocked with Lck peptide (ab52217).
IP Use a concentration of 20 - 25 µg/ml.

Target

  • FunctionTyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • Tissue specificityExpressed specifically in lymphoid cells.
  • Involvement in diseaseNote=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • Post-translational
    modifications
    Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • Cellular localizationCytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • IMD22 antibody
    • LCK antibody
    • Lck p56 antibody
    • LCK proto-oncogene, Src family tyrosine kinase antibody
    • LCK_HUMAN antibody
    • Leukocyte C-terminal Src kinase antibody
    • LSK antibody
    • Lymphocyte cell specific protein tyrosine kinase antibody
    • Lymphocyte cell-specific protein-tyrosine kinase antibody
    • Lymphocyte specific protein tyrosine kinase antibody
    • Membrane associated protein tyrosine kinase antibody
    • Oncogene lck antibody
    • P56 LCK antibody
    • p56(LSTRA) protein tyrosine kinase antibody
    • p56-LCK antibody
    • p56lck antibody
    • pp58 lck antibody
    • pp58lck antibody
    • Protein YT16 antibody
    • Proto oncogene tyrosine protein kinase LCK antibody
    • Proto-oncogene Lck antibody
    • Protooncogene tyrosine protein kinase LCK antibody
    • T cell specific protein tyrosine kinase antibody
    • T cell-specific protein-tyrosine kinase antibody
    • T lymphocyte specific protein tyrosine kinase p56lck antibody
    • Tyrosine-protein kinase Lck antibody
    • YT 16 antibody
    • YT16 antibody
    see all

Anti-Lck antibody images

  • Anti-Lck antibody (ab18896) at 1 µg/ml + Recombinant human Lck protein (ab79626) at 0.01 µg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • IHC image of ab18896 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18896, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Lck antibody (ab18896)

This product has been referenced in:
  • Shi Y  et al. Regulatory T cells protect mice against coxsackievirus-induced myocarditis through the transforming growth factor beta-coxsackie-adenovirus receptor pathway. Circulation 121:2624-34 (2010). Read more (PubMed: 20530002) »
  • Strasner AB  et al. The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane. J Immunol 181:3706-13 (2008). WB ; Human . Read more (PubMed: 18714047) »

See all 2 Publications for this product

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