The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 49 kDa.
Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages.
Expressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta.
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labeling LCLAT1 with ab175516 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-LCLAT1 antibody - C-terminal (ab175516)
Anti-LCLAT1 antibody - C-terminal (ab175516) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg
Secondary Anti-rabbit HRP conjugated antibody at 1/10000 dilution