Anti-LCLAT1 antibody - C-terminal (ab175516)

Overview

  • Product name
    Anti-LCLAT1 antibody - C-terminal
    See all LCLAT1 primary antibodies
  • Description
    Rabbit polyclonal to LCLAT1 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LCLAT1 aa 303-332 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6UWP7

  • Positive control
    • Mouse kidney tissue lysate, Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab175516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 49 kDa.

Target

  • Function
    Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages.
  • Tissue specificity
    Expressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta.
  • Pathway
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • Sequence similarities
    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • Domain
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1-acylglycerol-3-phosphate O-acyltransferase 8 antibody
    • 1-AGP acyltransferase 8 antibody
    • 1-AGPAT 8 antibody
    • 1AGPAT8 antibody
    • Acyl-CoA:lysocardiolipin acyltransferase 1 antibody
    • AGPAT8 antibody
    • ALCAT1 antibody
    • HSRG1849 antibody
    • lclat1 antibody
    • LCLT1_HUMAN antibody
    • LYCAT antibody
    • Lysocardiolipin acyltransferase 1 antibody
    • UNQ1849 antibody
    • UNQ1849/PRO3579 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labeling LCLAT1 with ab175516 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-LCLAT1 antibody - C-terminal (ab175516) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 49 kDa

References

ab175516 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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