Overview

  • Product name
  • Description
    Rabbit polyclonal to LCMT2
  • Specificity
    ab112562 is predicted to not cross-react with other LCMT protein family members. At least two isoforms of LCMT2 are known to exist; this antibody will detect only the long isoform.
  • Tested applications
    Suitable for: WB, ICC/IF, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (16 amino acids) near the N terminal of Human LCMT2.

  • Positive control
    • Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab112562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.
  • Pathway
    tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. LCMT family.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0547 antibody
    • LCMT 2 antibody
    • Lcmt2 antibody
    • LCMT2_HUMAN antibody
    • Leucine carboxyl methyltransferase 2 antibody
    • MGC9534 antibody
    • p21WAF1/CIP1 promoter interacting protein antibody
    • p21WAF1/CIP1 promoter-interacting protein antibody
    • PPM2 antibody
    • tRNA Wybutosine synthesizing protein 4 antibody
    • tRNA wybutosine synthesizing protein 4 homolog antibody
    • tRNA wybutosine-synthesizing protein 4 homolog antibody
    • tRNA yW synthesizing protein 4 antibody
    • tRNA yW synthesizing protein 4 homolog antibody
    • tRNA yW-synthesizing protein 4 homolog antibody
    • TYW4 antibody
    see all

Images

  • Immunofluorescence of LCMT2 in mouse brain tissue with ab112562 at 20 ug/mL.
  • All lanes : Anti-LCMT2 antibody (ab112562) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 76 kDa
  • Immunohistochemistry of LCMT2 in mouse brain tissue with ab112562 at 5 ug/mL.

References

ab112562 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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