Recombinant fragment: SDEEIQDVSG TWYLKAMTVD REFPEMNLES VTPMTLTTLE GGNLEAKVTM LISGRCQEVK AVLEKTDEPG KYTADGGKHV AYIIRSHVKD HYIFYCE, corresponding to amino acids 24-121 of Human LCN1 (NP_002288) with a tag of 26 kDa. Total MW of immunogen is 37 kDa.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).
Use at an assay dependent dilution. Detection limit for this antibody is ~3ng/ml as a capture antibody.
FunctionCould play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
Tissue specificityMainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.