Overview

  • Product nameAnti-LCN1 antibody
    See all LCN1 primary antibodies
  • Description
    Mouse monoclonal to LCN1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: SDEEIQDVSG TWYLKAMTVD REFPEMNLES VTPMTLTTLE GGNLEAKVTM LISGRCQEVK AVLEKTDEPG KYTADGGKHV AYIIRSHVKD HYIFYCE, corresponding to amino acids 24-121 of Human LCN1 (NP_002288) with a tag of 26 kDa. Total MW of immunogen is 37 kDa.

  • Positive control
    • Transfected 293T cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).
ELISA Use at an assay dependent dilution. Detection limit for this antibody is ~3ng/ml as a capture antibody.

Target

  • FunctionCould play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
  • Tissue specificityMainly expressed in lachrymal and salivary glands. Also expressed in the prostate.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • LCN1 antibody
    • LCN1_HUMAN antibody
    • Lipocalin 1 (protein migrating faster than albumin, tear prealbumin) antibody
    • Lipocalin 1 like 2 antibody
    • Lipocalin-1 antibody
    • PMFA antibody
    • Tear lipocalin antibody
    • Tear prealbumin antibody
    • Tlc antibody
    • TP antibody
    • VEG protein antibody
    • VEGP antibody
    • Von Ebner gland protein antibody
    see all

Anti-LCN1 antibody images

  • Anti-LCN1 antibody (ab76611) at 1/500 dilution + immunogen at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 19 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-LCN1 antibody (ab76611) at 1/500 dilution

    Lane 1 : LCN1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 19 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Detection limit for this antibody is ~3ng/ml as a capture antibody.

References for Anti-LCN1 antibody (ab76611)

This product has been referenced in:
  • Setälä NL  et al. Interaction of phospholipid transfer protein with human tear fluid mucins. J Lipid Res 51:3126-34 (2010). WB . Read more (PubMed: 20724654) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"