Overview

  • Product nameAnti-LCT antibody
  • Description
    Rabbit polyclonal to LCT
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence corresponding to amino acids 180-328 of Human LCT.

  • Positive control
    • Human stomach tissue
  • General notesStore product undiluted. The antibody solution should be gently mixed before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab121260 was affinity purified using the PrEST antigen as affinity ligand and is mono-specific.
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab121260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform Heat Induced Epitope Retrieval (HIER) at pH 6.

Target

  • FunctionLPH splits lactose in the small intestine.
  • Tissue specificityIntestine.
  • Involvement in diseaseDefects in LCT are the cause of congenital lactase deficiency (COLACD) [MIM:223000]; also known as hereditary alactasia or disaccharide intolerance II. Congenital lactase deficiency is a an autosomal recessive, rare and severe gastrointestinal disorder. It is characterized by watery diarrhea in infants fed with breast milk or other lactose-containing formulas. An almost total lack of LCT activity is found in jejunal biopsy material of patients with congenital lactase deficiency. Opposite to congenital lactase deficiency, adult-type hypolactasia, also known as lactose intolerance, is the most common enzyme deficiency worldwide. It is caused by developmental down-regulation of lactase activity during childhood or early adulthood. The decline of lactase activity is a normal physiological phenomenon; however, the majority of Northern Europeans have the ability to maintain lactase activity and digest lactose throughout life (lactase persistence). The down-regulation of lactase activity operates at the transcriptional level and it is associated with a noncoding variation in the MCM6 gene, located in the upstream vicinity of LCT.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 1 family.
  • DomainThe sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.
  • Cellular localizationApical cell membrane. Brush border.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAC antibody
    • Lactase antibody
    • Lactase phlorizin hydrolase 1 antibody
    • Lactase phlorizin hydrolase antibody
    • Lactase-glycosylceramidase antibody
    • Lct antibody
    • LPH antibody
    • LPH_HUMAN antibody
    • LPH1 antibody
    • Phlorizin hydrolase antibody
    see all

Anti-LCT antibody images

  • ab121260, at 1/300 dilution, staining LCT in paraffin-embedded Human stomach tissue by Immunohistochemistry, showing strong cytoplasmic positivity in glandular cells.

References for Anti-LCT antibody (ab121260)

ab121260 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121260.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"