Overview

  • Product name
    Anti-LCT antibody
  • Description
    Rabbit polyclonal to LCT
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence corresponding to amino acids 180-328 of Human LCT.

  • Positive control
    • Human stomach tissue
  • General notes
    Store product undiluted. The antibody solution should be gently mixed before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab121260 was affinity purified using the PrEST antigen as affinity ligand and is mono-specific.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab121260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform Heat Induced Epitope Retrieval (HIER) at pH 6.

Target

  • Function
    LPH splits lactose in the small intestine.
  • Tissue specificity
    Intestine.
  • Involvement in disease
    Defects in LCT are the cause of congenital lactase deficiency (COLACD) [MIM:223000]; also known as hereditary alactasia or disaccharide intolerance II. Congenital lactase deficiency is a an autosomal recessive, rare and severe gastrointestinal disorder. It is characterized by watery diarrhea in infants fed with breast milk or other lactose-containing formulas. An almost total lack of LCT activity is found in jejunal biopsy material of patients with congenital lactase deficiency. Opposite to congenital lactase deficiency, adult-type hypolactasia, also known as lactose intolerance, is the most common enzyme deficiency worldwide. It is caused by developmental down-regulation of lactase activity during childhood or early adulthood. The decline of lactase activity is a normal physiological phenomenon; however, the majority of Northern Europeans have the ability to maintain lactase activity and digest lactose throughout life (lactase persistence). The down-regulation of lactase activity operates at the transcriptional level and it is associated with a noncoding variation in the MCM6 gene, located in the upstream vicinity of LCT.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 1 family.
  • Domain
    The sequence exhibits 4 regions (I-IV) of internal homology; therefore LPH might have evolved by two cycles of partial gene duplication.
  • Cellular localization
    Apical cell membrane. Brush border.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAC antibody
    • Lactase antibody
    • Lactase phlorizin hydrolase 1 antibody
    • Lactase phlorizin hydrolase antibody
    • Lactase-glycosylceramidase antibody
    • Lct antibody
    • LPH antibody
    • LPH_HUMAN antibody
    • LPH1 antibody
    • Phlorizin hydrolase antibody
    see all

Images

  • ab121260, at 1/300 dilution, staining LCT in paraffin-embedded Human stomach tissue by Immunohistochemistry, showing strong cytoplasmic positivity in glandular cells.

References

ab121260 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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